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- 천연물 분리 분석, 분획 및 농축
천연물 분리 분석, 분획 및 농축
Automated Fractionation System (Sepiatec)
Crude Biological Extract로 부터 완전 자동으로 HPLC-SPE Coupling System 을 이용하여 분리한 후
Fraction Collector을 이용하여 96 well plate 또는 test tube에 분취 가능
- HPLC/SPE Coupling 을 이용한 완전 자동 분리, 분취 시스템
- 천연물, Crude biological extracts 분리 분석
- 약효 탐색을 위한 다량의 샘플 확보 용이
- 최적화된 Column switching 기술로 복잡한 혼합물로부터 독립된 성분으로 분리
- 전문화된 기술 적용으로 신약 개발, 천연물 분야에 최적의 방법 제시
- 극성 화합물부터 비극성 화합물까지 전 부분을 한번에 분리, 분취 가능
1. Sepbox2D-250
| Modes of operation | Medium to non-polar, Polar Setup (optional) |
|---|---|
| Sample size | Max. 250 mg of extract |
| Pumps | 2 HPLC pumps |
| Columns | 1 injection column, 1 main separation column, 3 second separation step, 18 trap columns |
| Detection | UV detector, ELSD detector (optional) |
| Fraction collector | Fraction collector with microtiter plates, 24 deep-well, 48 or 96 well plates |
| Modes of collection | Time and peak based |
2. Sepbox2D-2000
| Gradient pump | 100 ml head, 400 bar, ternary gradient |
|---|---|
| Water pump | 100 ml head, 400 bar |
| Valves | 13 electrically actuated valves |
| njection | Up to 2,000 mg of extract with injection column technique or liquid injection using a 1ml loop |
| 1st separation column | 1 column, 150 x 32 mm |
| 2nd separation columns | 6 columns, 250 x 16 mm |
| Trap columns | 18 columns, 30 x 32 mm |
| UV detector | 3 mm flow cell |
| ELSD detector | Splitted flow for ELSD |
| Fraction collector | 12 racks, 48 vials each, max. capacity 60 ml/vial, max. 576 fractions |
3. Parallel HPLC / SFC
| System | 8 channel HPLC system with Flow Control unit |
|---|---|
| Operating mode | Up to 8 columns in parallel |
| Number of solvents | Up to 24 with optional Eluent Rack |
| Pump | 10 ml pump head, incl. low pressure gradient module and online-degaser |
| Operating pressure | Up to 400 bar |
| Flow Control | Active flow regulation in 8 channels, flow rates 0.25 to 1.5 ml per channel, automatic purge valve |
| Autosampler | Screening version, parallel injection of 1 sample at a time onto 8 columns |
| Columns | Up to 250 mm length, any vendor |
| Column Oven | (Optional) 8 columns, ambient temperature to 70°C |
| Detector | 8x Diode Array Detector, parallel detection of the entire spectrum (200 – 600 nm) |
4. The Spematrix
| System | 8 channel SFC system with Flow Control unit |
|---|---|
| Operating mode | Up to 8 columns in parallel |
| Number of solvents | Up to 18 solvents with optional Sepmatix Eluent Rack |
| CO2 pump | 100 ml pump head, 400 bar |
| Modifier pump | 100 ml pump head, 400 bar |
| Operating pressure | Up to 300 bar, adjustable online back pressure control |
| Columns | Up to 250 mm length, any vendor |
| Column Oven | (Optional) 8 columns, ambient temperature to 70° C |
| Detector | 8x Diode Array Detector, 8x 10 mm flowcells, pressure stable |
5. Preparative SFC5
| Operating mode | 1 analytical or semi-preparative column |
|---|---|
| Solvents | CO2 and organic solvents |
| Number of modifier solvents | 1 standard, max. 4 solvents with optional selection valve |
| Total flow rate | 50 ml/min at a modifier portion of 40 % |
| CO2 pump | 30 ml pump head, 400 bar |
| Modifier pump | 30 ml pump head, 400 bar |
| Add - on pump | 10 ml pump head, 400 bar (optional) |
| Operating pressure | Up to 300 bar, back pressure regulator online adjustable |
6. Prep SFC M5
| Solvents | CO2 and organic solvents |
|---|---|
| Number of modifier solvents | 1 standard, max. 4 with optional selection valve |
| Operating pressure | Up to 300 bar, back pressure regulator online adjustable |
| Autosampler | 2 standard microtiter plates or 2 deep well MTP with 24 wells 10 ml each, racks with 60 vials 1.5 ml or 24 vials 5 ml each |
| UV detector | Wavelength range 190 to 500 nm |
| Other detectors | DAD, ELSD or mass spectrometer optional |
7. Prep SFC 250
| Operating mode | 1 semi-preparative or preparative column |
|---|---|
| Solvents | CO2 and organic solvents |
| Number of modifier solvents | 1 standard, 4 with optional solvent selection valve |
| CO2 pump | 150 ml pump head, 400 bar |
| Modifier pump | 150 ml pump head, 400 bar |
| Add-on pump | 30 ml pump head, 400 bar (optional) |
| Operating pressure | Up to 300 bar, back pressure regulator online adjustable |
8. Prep SFC 660
| Operating mode | 1 preparative column |
|---|---|
| Solvents | CO2 and organic solvents |
| Number of modifier solvents | 3 solvents, mixable, solvent selection valves included |
| Total flow rate | 667 ml/ min at a modifier portion of 40 % |
| Pump 1, CO2 | 400 ml pump head, 400 bar |
| Pump 2, modifier | 250 ml pump head, 400 bar |
| Pump 3, modifier/ add-on | 150 ml pump head, 400 bar |
| Operating pressure | Up to 300 bar, back pressure regulator online adjustable |
| UV detector | Wavelength range 190 to 500 nm |
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